Adaptation kinetics in bacterial chemotaxis

Cells of Escherichia coli, tethered to glass by a single flagellum, were subjected to constant flow of a medium containing the attractant alpha-methyl-DL-aspartate. The concentration of this chemical was varied with a programmable mixing apparatus over a range spanning the dissociation constant of the chemoreceptor at rates comparable to those experienced by cells swimming in spatial gradients. When an exponentially increasing ramp was turned on (a ramp that increases the chemoreceptor occupancy linearly), the rotational bias of the cells (the fraction of time spent spinning counterclockwise) changed rapidly to a higher stable level, which persisted for the duration of the ramp. The change in bias increased with ramp rate, i.e., with the time rate of change of chemoreceptor occupancy. This behavior can be accounted for by a model for adaptation involving proportional control, in which the flagellar motors respond to an error signal proportional to the difference between the current occupancy and the occupancy averaged over the recent past. Distributions of clockwise and counterclockwise rotation intervals were found to be exponential. This result cannot be explained by a response regular model in which transitions between rotational states are generated by threshold crossings of a regular subject to statistical fluctuation; this mechanism generates distributions with far too many long events. However, the data can be fit by a model in which transitions between rotational states are governed by first-order rate constants. The error signal acts as a bias regulator, controlling the values of these constants

Criminology as an educational radio topic.

Thesis (M.S.)--Boston Universit

Coordination of flagella on filamentous cells of Escherichia coli

Video techniques were used to study the coordination of different flagella on single filamentous cells of Escherichia coli. Filamentous, nonseptate cells were produced by introducing a cell division mutation into a strain that was polyhook but otherwise wild type for chemotaxis. Markers for its flagellar motors (ordinary polyhook cells that had been fixed with glutaraldehyde) were attached with antihook antibodies. The markers were driven alternately clockwise and counterclockwise, at angular velocities comparable to those observed when wild-type cells are tethered to glass. The directions of rotation of different markers on the same cell were not correlated; reversals of the flagellar motors occurred asynchronously. The bias of the motors (the fraction of time spent spinning counterclockwise) changed with time. Variations in bias were correlated, provided that the motors were within a few micrometers of one another. Thus, although the directions of rotation of flagellar motors are not controlled by a common intracellular signal, their biases are. This signal appears to have a limited range

Supporting public availability and accessibility with Elvin: experiences and reflections.

We provide a retrospective account of how a generic event notification service called Elvin and a suite of simple client applications: CoffeeBiff, Tickertape and Tickerchat, came to be used within our organisation to support awareness and interaction. After overviewing Elvin and its clients, we outline various experiences from data collated across two studies where Elvin and its clients have been used to augment the workaday world to support interaction, to make digital actions visible, to make physical actions available beyond the location of action, and to support content and socially based information filtering. We suggest there are both functional and technical reasons for why Elvin works for enabling awareness and interaction. Functionally, it provides a way to produce, gather and redistribute information from everyday activities (via Elvin) and to give that information a perceptible form (via the various clients) that can be publicly available and accessible as a resource for awareness. The integration of lightweight chat facilities with these information sources enables awareness to easily flow into interaction, starting to re-connect bodies to actions, and starting to approximate the easy flow of interaction that happens when we are co-located. Technically, the conceptual simplicity of the Elvin notification, the wide availability of its APIs, and the generic functionality of its clients, especially Tickertape, have made the use of the service appealing to developers and users for a wide range of uses

Critical Inquiry, Conceptual Clarity, and Contextual Limits. A Response to “Re-centering Civics: A Framework for Building Dispositions and Action Opportunities”

In Re-centering Civics: A Framework for Building Dispositions and Action Opportunities, the authors presented a framework to help social studies teachers in any subject or grade level re-center civic education. The authors’ article draws from the C3 Framework and C3Teachers.org to offer six civic dispositions teachers might focus on cultivating with their students, and the article highlights ways in which student engagements with any historical inquiry might be steered toward real-world civic action. In this response, we underscore the strengths of Re-centering Civics while also outlining a necessary, critical attention to the concepts undergirding the authors’ framework. Our response builds from Re-centering Civics by offering examples of how the concepts at play in the initial article might be reconfigured, how teacher questioning can be made more critical, how issues of diversity and power can be more effectively attended to, and how the everyday, contextual limitations of teachers might affect their ability to carry out this framework. Our response aims to strengthen the authors’ admirable project, one we are fully aligned with: integrating thoughtful, critical, and deliberate civic education—and meaningful action—into social studies education writ large

Broadcast Guidance of Multi-Agent Systems

We consider the emergent behavior of a group of mobile agents guided by an exogenous broadcast signal. The agents’ dynamics is modelled by single integrators and they are assumed oblivious to their own position, however they share a common orientation (i.e. they have compasses). The broadcast control, a desired velocity vector, is detected by arbitrary subgroups of agents,that upon receipt of the guidance signal become "ad-hoc" leaders. The control signal and the set of leaders are assumed to be constant over some considerable intervals in time. A system without "ad-hoc" leaders is referred to as autonomous. The autonomous rule of motion is identical for all agents and is a gathering process ensuring a cohesive group. The agents that become leaders upon receipt of the exogenous control add the detected broadcast velocity to the velocity vector dictated by the autonomous rule of motion. This paradigm was considered in conjunction with several models of cohesive dynamics, linear and non-linear, with fixed inter-agent interaction topology, as well as systems with neighborhood based topology determined by the inter-agent distances. The autonomous dynamics of the models considered provides cohesion to the swarm, while, upon detection of a broadcast velocity vector, the leaders guide the group of agents in the direction of the control. For each local cohesion interaction model we analyse the effect of the broadcast velocity and of the set of leaders on the emergent behavior of the system. We show that in all cases considered the swarm moves in the direction of the broadcast velocity signal with speed set by the number of agents receiving the control and in a constellation determined by the model and the subset of "ad-hoc" leaders. All results are illustrated by simulations

A molecular simulation analysis of producing monatomic carbon chains by stretching ultranarrow graphene nanoribbons

Atomistic simulations were utilized to develop fundamental insights regarding the elongation process starting from ultranarrow graphene nanoribbons (GNRs) and resulting in monatomic carbon chains (MACCs). There are three key findings. First, we demonstrate that complete, elongated, and stable MACCs with fracture strains exceeding 100% can be formed from both ultranarrow armchair and zigzag GNRs. Second, we demonstrate that the deformation processes leading to the MACCs have strong chirality dependence. Specifically, armchair GNRs first form DNA-like chains, then develop into monatomic chains by passing through an intermediate configuration in which monatomic chain sections are separated by two-atom attachments. In contrast, zigzag GNRs form rope-ladder-like chains through a process in which the carbon hexagons are first elongated into rectangles; these rectangles eventually coalesce into monatomic chains through a novel triangle-pentagon deformation structure under further tensile deformation. Finally, we show that the width of GNRs plays an important role in the formation of MACCs, and that the ultranarrow GNRs facilitate the formation of full MACCs. The present work should be of considerable interest due to the experimentally demonstrated feasibility of using narrow GNRs to fabricate novel nanoelectronic components based upon monatomic chains of carbon atoms.Comment: 11 pages, 6 figures, Nanotechnology accepted versio

A Validated Reversed-Phase HPLC Method for the Determination of Atorvastatin Calcium in Tablets

A Reversed-Phase Liquid Chromatographic (RP-LC) assay method was developed for the quantitative determination of atorvastatin calcium in the presence of its degradation products. The assay involved an isocratic elution of atorvastatin calcium in a LiChroCARTR 250*4 mm HPLC Cartridge LiChrospherR 100 RP-18 (5 μm) column using a mobile phase consisting of 0.1% acetic acid solution: acetonitrile (45:55, v/v), pH = 3.8. The flow rate was 0.8 mL/min and the analytes monitored at 246 nm. The assay method was found to be linear from 8.13 to 23.77 μg/mL. All the validation parameters were within the acceptance range. The developed method was successfully applied to estimate the amount of atorvastatin calcium in tablets.Fil: Simionato, Laura Daniela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Ferello, L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Stamer. S.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Repetto, M. F.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Zubata, P. D.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; ArgentinaFil: Segall, Adriana Ines. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentin